Expression plasmid of an aminoacyl-tRNA synthetase for AzoPhe
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1164
Illegal BamHI site found at 1170
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 1196
Illegal NgoMIV site found at 1656
- 1000COMPATIBLE WITH RFC
The coding sequence of the synthetase has to be inserted into a linearized ONBY-Part. For example per Gibson Assembly of a gene synthesis with matching overahangs.
It can be obtained by cloning the CDS in the linearized ONBY-part as shown in Figure 1.
Collaboration with CU Boulder, Schultz lab, Addgene