Coding

Part:BBa_K2201207:Design

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Expression plasmid of an aminoacyl-tRNA synthetase for AzoPhe

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
    Illegal BamHI site found at 1170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1656
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of the synthetase has to be inserted into a linearized ONBY-Part. For example per Gibson Assembly of a gene synthesis with matching overahangs.

It can be obtained by cloning the CDS in the linearized ONBY-part as shown in Figure 1.

Figure 1: History of the plasmid construction of K2201207.

Source

Collaboration with CU Boulder, Schultz lab, Addgene

References