Composite

Part:BBa_K2201204:Design

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Tyrosyl tRNA/aminoacyl-synthetase for the incorporation of L‑(7‑hydroxycoumarin‑4

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1223
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene synthesisi was inserted via site directed mutagenesis.


Source

Wang, J., Xie, J., Schultz, P. G.(2006). A Genetically Encoded Fluorescent Amino Acid. American Chemical Society.128:8738-8739, ordered by gene synthesis.

References