Part:BBa_K220002

GSMT is a plasmid gene (854 bp) that codes for the enzyme that catalyze the first reaction in the salt tolerant pathway that we are interested. GSMT stands for Glycine Sarcosine N-Methyl Transferase. It catalyzes the conversion from glycine to sarcosine, using SAM as a methyl donor. In our project, we have this GSMT synthesized using MR. Gene. Naturally, this gene can be found in Galdieria sulphuraria, an extremephile that establishes high resistance to osmotic stress as well as acidic, thermal stresses and toxic metals. We PCR amplified gene GSMT with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.

Modeling of [http://2009.igem.org/Team:Wisconsin-Madison/GSMT GSMT]

Modeling of [http://2009.igem.org/Team:Wisconsin-Madison/GSMT%2BSDMT GSMT and SDMT]

Reference: A Nyyssölä, T Reinikainen, M Leisola Characterization of Glycine Sarcosine N-Methyltransferase and Sarcosine Dimethylglycine N-Methyltransferase http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92834/?tool=pubmed