Part:BBa_K2197401
smUOX downstream a repressor operator site
Design This is a composite part encoding hucO(BBa_K2197303) and smUOX(BBa_K2197402) gene. smUOX is a human gene for urate oxidase, which is an enzyme that digest uric acid. The circuit uses a bacterial transcriptional repressor (HucR) that binds a DNA sequence motif (hucO) in the absence of uric acid. When uric acid is present, HucR dissociates from hucO motif, thereby allowing expression of a downstream smUOX gene. It is hoped that the expression of the downstream smUOX gene is regulated by the concentration of uric acid in a proportional manner so that uric acid is cleared out.
Mechanism of BBa_K2197400
Expression of a strong repressor (mUTS)
Operator site and smUOX
Expression without uric acid (-UA)
mUTS binds with HucO and repress the expression of downstream smUOX.
expression with uric acid (+UA)
mUTS dissociates with HucO to different extents according to the concentration of uric acid, thus expressing downstream smUOX at different levels.
Kemmer, Christian & Gitzinger, Marc & Daoud-El Baba, Marie & Djonov, Valentin & Stelling, Jörg & Fussenegger, Martin. (2010). Self-sufficient control of urate homeostasis in mice by a synthetic circuit. Nature biotechnology. 28. 355-60. 10.1038/nbt.1617.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 722
Illegal PstI site found at 944 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 815
Illegal PstI site found at 722
Illegal PstI site found at 944 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 722
Illegal PstI site found at 944 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 722
Illegal PstI site found at 944 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 83
Illegal SapI.rc site found at 1033
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