Primer

Part:BBa_K2184040

Designed by: Margalit Mualem   Group: iGEM16_Danci-K8   (2016-09-30)


Reverse primer

Engineered Reverse primer with SpeI enzyme site by APE software

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None