Designed by: Chern Juin Ong, Alex Mayorov, Mie Monti Group: iGEM16_Cambridge-JIC (2016-10-11)
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal BamHI site found at 3379
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Two Guanine nucleotides added to downstream of the CDS to create a Gly-Ser linker is advised by Dr N. Patron (Earlham Institute), which allows for the creation of fusion proteins. The stop codon is also removed for this purpose. Multiple Bpe1 sites within the CDS was removed without changing the amino acid it translates to.