Coding

Part:BBa_K2148009

Designed by: Chern Juin Ong   Group: iGEM16_Cambridge-JIC   (2016-10-10)


C.reinhardtii CO green fluorescent protein

A coding DNA sequence for green fluorescent protein (GFP) flanked by fusion site for a CDS/CTAG Phytobrick, which can be used as a reporter gene.

Usage and Biology

Green fluorescent protein can be used as a reporter. Coded as a CDS/CTAG Phytobrick conforming to the Phytobrick standard, this part can be used with other level-0 Phytobricks to form the desired transcriptional unit.

It is an optimised form of the original GFP gene, BBa_E0040, which we codon-optimised for the chloroplasts of Chlamydomonas reinhardtii.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

References

Davis, S.J. & Vierstra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182

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Parameters
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