C.reinhardtii CO green fluorescent protein
A coding DNA sequence for green fluorescent protein (GFP) flanked by fusion site for a CDS/CTAG Phytobrick, which can be used as a reporter gene.
Usage and Biology
Green fluorescent protein can be used as a reporter. Coded as a CDS/CTAG Phytobrick conforming to the Phytobrick standard, this part can be used with other level-0 Phytobricks to form the desired transcriptional unit.
It is an optimised form of the original GFP gene, BBa_E0040, which we codon-optimised for the chloroplasts of Chlamydomonas reinhardtii.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Davis, S.J. & Vierstra, R.D. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol (1998) 36: 521. doi:10.1023/A:1005991617182