Strong promoter/RBS for MAHS
- 10COMPATIBLE WITH RFC
- 12INCOMPATIBLE WITH RFCIllegal NheI site found at 7
Illegal NheI site found at 30
- 21INCOMPATIBLE WITH RFCIllegal BamHI site found at 508
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We have attempted to maximize expression of MAHS.
The coding sequence of this composite part is a member of a class of intrinsically disordered proteins called the Late Embryonic Abundant (LEA) proteins that become ordered when desiccated, thereby stabilizing DNA and protein cell components. This tardigrade unique protein is found in the mitochondria of tardigrade cells. When cloned into humans, they confer protection against osmotic stress as shown in the paper "Novel Mitochondria-Targeted Heat-Soluble Proteins Identified in the Anhydrobiotic Tardigrade Improve Osmotic Tolerance of Human Cells" by Sae Tanaka et al. 2015 Feb 12. It was taken from the genome of Ramazzottius varieornatus and optimized by the IDT Codon Optimization Tool for E. Coli K12.