Composite

Part:BBa_K2128006

Designed by: David Sanderson   Group: iGEM16_Genspace   (2016-08-13)


RFP with H. Dujardini ACTN Promoter, RBS and Terminator

This part encodes for a Red Fluorescent Protein in H. Dujardini and potentially other Tardigrade species.

The RFP in this part was taken from BBa_E1010, a "highly engineered mutant of red fluorescent protein from Discosoma striata". It was codon optimized Optimized for C. Elegans by IDT's Codon Optimization Tool.

The promoter, RBS and Terminator where taken from the H. Dujardini ACTN gene. It is a constitutive promoter that is on at all stages of development.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 716
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 716
    Illegal BglII site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 716
    Illegal AgeI site found at 766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI site found at 280


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Categories
Parameters
None