Composite

Part:BBa_K2121008

Designed by: Leslie Wang   Group: iGEM16_Yale   (2016-10-21)


Melibiose Inducible Promoter

Promoter regions in S. meliloti inducible by melibiose.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 142
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 274
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 142
    Illegal NheI site found at 4972
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 274
    Illegal PstI site found at 436
    Illegal NotI site found at 2846
    Illegal NotI site found at 4379
    Illegal NotI site found at 5669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 142
    Illegal BamHI site found at 1554
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 142
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 274
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 142
    Illegal SpeI site found at 110
    Illegal PstI site found at 39
    Illegal PstI site found at 274
    Illegal PstI site found at 436
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 682
    Illegal NgoMIV site found at 2291
    Illegal NgoMIV site found at 2465
    Illegal NgoMIV site found at 2515
    Illegal NgoMIV site found at 2519
    Illegal NgoMIV site found at 2546
    Illegal AgeI site found at 92
    Illegal AgeI site found at 5466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1521
    Illegal BsaI.rc site found at 793
    Illegal SapI site found at 708
    Illegal SapI site found at 6060
    Illegal SapI.rc site found at 5597


[edit]
Categories
Parameters
None