Coding

Part:BBa_K211002

Designed by: ZHONG Wanting   Group: iGEM09_HKUST   (2009-10-13)

RI7-odr10 chimeric GPCR

BBa_K211002 encodes a chimeric diacetyl-sensing GPCR which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the TM2-TM7 ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. It can be localized to the S. cerevisiae cell membrane and activate the yeast endogenous MAPK pathway upon binding to the ligand diacetyl.

Receptor.PNG

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/receptor
//chassis/eukaryote/yeast
//function/cellsignalling
Parameters
chassisyeast
familyGPCR
functiondiacetyl sensing GPCR
ligandsdiacetyl
originC.elegans ODR-10 and Rat RI7
tagFLAG