Designed by: Tom van Sonsbeek   Group: iGEM16_TU-Eindhoven   (2016-10-10)

Applications of BBa_K2065007

iGEM TU Eindhoven 2016

iGEM TU Eindhoven used this part in combination with CT52-SmallBiT (BBa_K2065000). These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised in figure 1. For more detailed information about the application of CT52-LargeBiT visit our wiki "".

Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52

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