- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 45
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 699
- 1000COMPATIBLE WITH RFC
A BamHI site was placed between the promoter and the RBS in order allow expression with other promoter This part was introduced in pSEVA424 plasmid in order to increase the lactate fraction in Pseudomonas putida.
The LDH* were synthesized from the genomic sequence of Lactobacillus delbrueckii 11842