RBS + Pct
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 688
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 1094
Illegal BsaI site found at 1565
We inserted non-coding scar sequences after RBS witch is close to the gene to prevent missing initial codons during translation.
The RBS were synthesized from the genomic sequence of P.putida, Pct gene were synthetised from genomic sequence of Clostridum propionicum.