Pseudomonas resinovorans PHA synthase 1
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 87
Illegal XhoI site found at 172
Illegal XhoI site found at 1030
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 1060
- 1000Illegal BsaI site found at 796
This is the DNA coding for the enzyme Polyhydroxyalkanoate synthase from Pseudomonas resinovorans. This sequence was codon optimized for Pseudomonas putida and four amino acid mutations were introduced to increase yields of Polylactic acid (PLA). Silent mutation were also introduced for removing restriction site. The iGEM Team Evry 2016 used this enzyme as the second enzyme in the pathway to produce PLA in Pseudomonas putida.
Pseudomonas resinovorans gDNA. This sequence was synthetized by IDT
Biosynthesis of Polylactic Acid and Its Copolymers using Evolved Propionate CoA Transferase and PHA Synthase
Taek Ho Yang,1 Tae Wan Kim,1 Hye Ok Kang,1 Sang-Hyun Lee,1 Eun Jeong Lee,1 Sung-Chul Lim,1 Sun Ok Oh,1 Ae-Jin Song,2 Si Jae Park,1 Sang Yup Lee2,3
Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.
Yang TH1, Jung YK, Kang HO, Kim TW, Park SJ, Lee SY.
Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.