Part:BBa_K2022005
alMGS, His-tag
This part is Acholeplasma laidlawii, Alpha-monoglucosyldiacylglycerol synthase (alMGS). It catalyses the insertion of lipids into the bacterial inner membrane; to relieve the stress of expansion, the membrane bulges in and releases those lipids in the form inner-membrane vesicles.
It is much the same as BBa_K2022004, but with a his tag just prior to the double stop; this is to enable easier antibody-based recognition e.g. for Western Blots
Usage and Biology
Unfortunately, we (UNSW 2016) overlooked this part having an EcoRI site at position 709-715, hence it lacks RFC10 compatibility. Future teams should definitely fix this error!
Aside from this, expression of this ORF is in E. coli thought to create cytoplasmic vesicles (Ge et al., 2013); these would be valuable for scaffolding chemical reactions or creating compartments of different chemistry inside E. coli.
We were in particular interested due to it being shown to localise to the outer-membrane fraction, too, and we thus hypothesised it could help stimulate lipid insertion into the outer-membrane (Ge et al., 2013), and thus, in turn, stimulate outer-membrane vesicle formation too.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 709
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 709
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 709
Illegal BglII site found at 491 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 709
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 709
Illegal AgeI site found at 768 - 1000COMPATIBLE WITH RFC[1000]
References
Ge, C., Gómez‐Llobregat, J., Skwark, M.J., Ruysschaert, J.M., Wieslander, Å. and Lindén, M., 2014. Membrane remodeling capacity of a vesicle‐inducing glycosyltransferase. FEBS Journal, 281(16), pp.3667-3684.
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