Coding

Part:BBa_K2020023

Designed by: Viviane Schink   Group: iGEM16_Aachen   (2016-10-13)


S. cerevisiae codon optimized Subtilisin E

optimization of a wild type Subtilisin E gene from Bacillus subtilis for Saccharomyces cerevisiae

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal BamHI site found at 193
    Illegal XhoI site found at 1091
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal AgeI site found at 305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 490


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Categories
Parameters
None