Hybrid promoter having multiple operator sites.
was designed according to the Lutz R and Bujard H.(Nucleic Acids Res. 1997 Mar 15;25(6):1203-10). The arrangement of the operator sites are the same as in BBa_K202000 except that tetO2 and LacO1 opeartor sequences are replaced by two nonbinding sequences (obtained from noncoding sequences of Fungus). This promoter has tetO2 sites which are having transverse mutation at position 5. The hybrid promoter is cloned upstream to the GFP (part Bba_E0240) in plasmid pSB1A3.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 192
- 1000COMPATIBLE WITH RFC
|n/a||Hybrid promoter having multiple operator sites. Promoter has tetO2 with mutation at position 5|