Part:BBa_K2018044
MaSp2 CD
Major ampullate spidroin 2 (MaSp2) is a gene for the dragline silk of the specie Nephilia Clavipes. This gene is divided into three parts, MaSp2 AB, MaSp2 BC and MaSp2 CA. This part is a modification of MaSp2 CA so it have different overhangs. This is a component which have the specifics overhangs of 5’-CGTG-3’ when treated with BsaI on 29bp. This makes it compatible with the MaSp2 BC sequence (K1763003). The sequence is 120 bps long. When treated with BsaI it also recognizes a sequence at 130bp and creates the overhang 5’-TAGC-3’ (notice the direction of the sequence). This sequence was made by iGEM 2016 team Southern Denmark in their project of Bacto-Aid. The part is designed for the technique Iterative Capped Assembly (ICA), which can elongate several steps of ligation creating your desired gene sequence, if overhangs are compatible. If you want to know more see our Wiki: http://2016.igem.org/Team:SDU-Denmark
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 115
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