35s:5'+ Ga20ox consense + SAGTI-Luciferase + Tnos
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1050
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1070
- 1000Illegal BsaI site found at 2765
Illegal BsaI.rc site found at 3052
It is important to notice that this device is modular. To insert the region of the gene of interest, this region must have the overhang 5'-AATG-3' and 5'-TTCG-3'
This part was obtained using GoldenBraid assembly. The Ga20ox consense sequence was obtained with oligonucleotide synthesis.