Coding

Part:BBa_K199130

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-03-29)


LuxI with 2 sites

This is LuxI optimized for E. coli. It contains 2 restriction sites (AscI and AsiSI). This facilitates the insertion of different frameshift mutations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None