Composite

Part:BBa_K1976024:Design

Designed by: Sonja Elbrich, Claudia Kreher, Patrick Kunzmann, Nina Kuschik-Maczollek, Bianca Reisinger, Sabine Schäfer, Inka Schröter, Viktoria Schuster   Group: iGEM16_TU_Darmstadt   (2016-10-13)


Experimental orthogonal pair for amber supression with OMT

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesis feasible by IDT, no amber stop codon for termination



Source

Methanocaldococcus jannaschii (optimized via directed evolution)


References