Coding

Part:BBa_K1923007

Designed by: Tianyang Mao   Group: iGEM16_Tsinghua   (2016-10-14)


Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene

NOTE
This part page no longer serves as the BioBrick for silver medal criteria. We instead would like to use this part as the evidence of improvement of previous part, and have created a new part page (https://parts.igem.org/Part:BBa_K1923011). However, since we have already saved this page in the judging form and shipped the plasmid, we do want to save the page as the evidence for successful shipment of BBa_K1923011, since they have identical sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4673
    Illegal XhoI site found at 220
    Illegal XhoI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 139


[edit]
Categories
Parameters
None