Coding

Part:BBa_K192001:Design

Designed by: Daniel Wong, Kenny Zhan, Jason An   Group: iGEM09_TorontoMaRSDiscovery   (2009-09-15)


CFP +tgt +lva

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797


Design Notes

We decided to position the LVA at the C-terminus of the construct since that is its natural position. However, the tgt extension sequence is also typically found at the C-terminus, so we're hoping that the shorter LVA sequence won't interfere too much with the binding with encapsulin. If necessary, it'll be possible to remove the LVA tag via PCR.


Source

Part was synthesized.

References