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Part:BBa_K1903003

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


Golden Gate Adapter

This parts is composed of a random sequence of 100 base pairs follow upstream and downstream by a BsaI restriction site. The fusion sites for both ends are part of the standard prefix and the suffix. The part has the advantage to be clone into a RFP plasmid with RFC 10 and then used as the vector for the Golden Gate reaction.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 107
    Illegal BsaI.rc site found at 1


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