Device

Part:BBa_K1886003

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


PcpcG2-sfGFP-ccaR

This circuit is composed of the inducible promoter cpcG2 and reporter gene and intracellular protein gene ccaR. ccaR could activate the cpcG2 promoter after being phosphorylated. cpcG2 could be activated by the phosphorylated ccaR protein, and induce the expression of reporter gene sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1398
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 269


Characterization

BACKGROUND

Overview

Registered Parts in our wiki is the subunit of the entire light-controlled system, each of which has no function. So I will introduce our light input part of our project after the combination of all the subunits in the page of part K1886006

RESULTS


Gel electrophoretic analysis

Fig.1 Fig.1 Gel electrophoretic analyses of PCR products




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