Coding

Part:BBa_K1847016

Designed by: Anna Fomitcheva, Lisa Baumgartner   Group: iGEM15_ETH_Zurich   (2015-09-16)

lldP-lldR

Regulatory protein lldR together with L-lactate permease (lldP).

Usage and Biology

LldP imports L-lactate, D-lactate and glycolate. It is probably driven by proton motive force. Sensitivity of the lldR promoter can be regulated by the expression of lldP which increases the import of lactate inside the cell1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 417
    Illegal AgeI site found at 880
    Illegal AgeI site found at 1318
    Illegal AgeI site found at 1477
    Illegal AgeI site found at 1489
    Illegal AgeI site found at 2228
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Biochemical and Biophysical Research Communications 290, 824–829 (2002)


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Categories
Parameters
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