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Applications of BBa_K1829002
pFig2c is an alpha factor responsive promoter that can be used to activate gene transcription in MATa S. Cerevisiae cells. It is activated by the presence of mating factor alpha, a part of the S. Cerevisiae mating pathway. Unfortunately it is not a very "strong" promoter, resulting in only 5-fold activation.
We cloned pFig2c driving production of RFP (red fluorescence protein) into S. Cerevisiae cells, in order to use RFP as a reporter for the activity of pFig2c. Using flow cytometry we measured the fluorescence of cells induced with varying levels of mating factor alpha