Designed by: Nicholas Suen Group: iGEM15_UCSF (2015-09-09)
pFig2c: Mating factor alpha responsive promoter
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 431
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 125
Illegal BsaI.rc site found at 564
We changed nucleeotide 22 from adenine to thymine in order to eliminate the illegal Pst1 cut site.
pFig2c is found in the genomic sequence of MATa S. cerevisiae.