Coding

Part:BBa_K1823005

Designed by: Isyatul Azizah   Group: iGEM15_UB_Indonesia   (2015-09-18)
Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: polyprotein glycoprotein

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


sE protein DENV2

This is a recombinant envelope glycoprotein of DENV1 (Dengue virus serotype 1). This part has signal peptide that will direct the protein into endoplasmic reticulum to complete the folding mechanism. It is used to confirm if the EDE1C8 scFv antibody secreted from E. coli work well.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 761
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 907
    Illegal AgeI site found at 970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1052


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Categories
Parameters
None