Coding

Part:BBa_K1823004

Designed by: Isyatul Azizah   Group: iGEM15_UB_Indonesia   (2015-09-18)
Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: polyprotein glycoprotein

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


sE protein DENV1

This is a recombinant envelope glycoprotein of DENV1 (Dengue virus serotype 1). This part has signal peptide that will direct the protein into endoplasmic reticulum to complete the folding mechanism. It is used to confirm if the EDE1C8 scFv antibody secreted from E. coli work well.

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 730
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 730
    Illegal NgoMIV site found at 1213
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 529
    Illegal SpeI site found at 730


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Categories
Parameters
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