mCherry (rights owned by Clontech)
- 10Illegal PstI site found at 352
- 12Illegal PstI site found at 352
- 21COMPATIBLE WITH RFC
- 23Illegal PstI site found at 352
- 25Illegal PstI site found at 352
- 1000Illegal PstI site found at 352
PstI site within the mCherry sequence - has to be assembled using BioBrick assembly in such an order that any successive digestions will not require PstI, otherwise the mCherry sequence will be cut as well as the site of interest. Alternatively, the assembly can be performed using non-standard BioBrick assembly.
N.B. This part has not been submitted to the registry, as the rights to mCherry are commercially owned by Clontech. Academic researchers are welcome to use this part, subject to a material transfer agreement from the supplying university, providing that the DNA will not be used for commercial profit or gain. The sequence provided on this registry page has been provided with express permission from Clontech.
pmCherry vector, Clontech
Shaner et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein. Nat Biotechnol (2004) vol. 22 (12) pp. 1567-1572