Regulatory

Part:BBa_K180000

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-12)

Hybrid promoter (trp & lac regulated -- tac pR)

The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences by a further PCR with specially designed primers.

[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25


Usage and Biology

The Ptac promoter sequence here is preceeded by a coding region for lacIq; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state. Ptac is also useful compared to it's single parent promoters trp pR and lac pR, as it has stronger binding affinity than either of its parent promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
n/aHybrid promoter (trp & lac regulated -- tac pR)