Coding

Part:BBa_K1774001

Designed by: HKU iGEM 2015   Group: iGEM15_Hong_Kong_HKU   (2015-09-18)


Cas9 (optimized for expression in E. coli)

This part code for cas9 originated from S. pyogenes SF 370 with codon optimized for expression in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3512


Improvement

Team Utrecht 2017 improved this part by codon optimizing for expression in human cell lines and allowing the protein to be secreted into the medium. See http://parts.igem.org/Part:BBa_K2351013

[edit]
Categories
//function/crispr/cas9
Parameters
None