Device

Part:BBa_K177005

Designed by: Michael Lower   Group: iGEM09_Warsaw   (2009-02-18)


Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


Bistable_1

Bistable switch for lysteriolysin/invasin expression control

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6521
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3080
    Illegal NgoMIV site found at 7425
    Illegal AgeI site found at 2589
    Illegal AgeI site found at 2913
    Illegal AgeI site found at 3048
    Illegal AgeI site found at 3126
    Illegal AgeI site found at 4635
    Illegal AgeI site found at 5139
    Illegal AgeI site found at 8273
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9093


[edit]
Categories
Parameters
None