Device

Part:BBa_K175043

Designed by: Tim_Weenink   Group: iGEM09_TUDelft   (2009-10-11)


Inducible Self Destructive Plasmid + inducible GFP-LVA reporter

Inducible Self Destructive Plasmid, consisting of IPTG inducible I-SceI homing endonuclease + inducible GFP-LVA reporter gene with an I-SceI restiction site integrated into the sequence between the promoter and the RBS. And an additional one between the GFP coding sequence and the TetR generator sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2190
    Illegal NheI site found at 2213
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1885


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Parameters
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