Coding

Part:BBa_K1732027:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


GLuc-His

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

6X-His tag for easier protein purification. Sequence was codon optimized for E.coli via IDT codon optimization tools.


Source

BBa_K1732003

References