Coding

Part:BBa_K1732025:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


RFPCO-His

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 552
    Illegal AgeI site found at 664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His tag for easier protein purification and codon optimized for E.coli.


Source

BBa_1491008

References