Composite

Part:BBa_K173006

Designed by: Lorenzo Pasotti, Letizia Diamante, Susanna Schiavi, Paolo Magni   Group: iGEM09_UNIPV-Pavia   (2009-10-15)

tetR inverter - beta-galactosidase generator

This composite part can be used as a measurement system for beta-galactosidase expression: tetR promoter (BBa_R0040) acts as a constitutive promoter because tetR (BBa_C0040) is not expressed and so lacZ (BBa_I732005) is always transcribed.

If a promoter is assembled upstream, tetR expression can be modulated by the strength of the promoter and this device can be induced by aTc which binds tetR and inactivates it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None