Device

Part:BBa_K1718002

Designed by: Daren Kraft and Josephina Hendrix   Group: iGEM15_CU_Boulder   (2015-09-13)


Genetic Switch for Bxb1 invertase

This part consists of a strong Anderson promoter and a terminator flanked by attB and attP, the recognition sites for BxbI. In its off position, the terminator prevents expression of the downstream part. If the BxbI invertase is expressed, it will recognize the attB and attP sites and invert the DNA; thus, flipping the terminator so polymerase can pass through the gate. The Bxb1 invertase flips DNA using the attB and attP sites. There is a Bxb1 excisionase that can flip the DNA back.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 55
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84
    Illegal BsaI.rc site found at 245


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Categories
Parameters
None