Composite

Part:BBa_K1688000:Design

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)


Mono-rhamnolipid gene (inc RBS, J23101 promoter)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 130
    Illegal BamHI site found at 690
    Illegal XhoI site found at 866
    Illegal XhoI site found at 2180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1794
    Illegal NgoMIV site found at 1907
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 355
    Illegal BsaI site found at 1423
    Illegal BsaI.rc site found at 539


Design Notes

no specific consideration


Source

2015 DNA distribution kit

References