Composite

Part:BBa_K1677025

Designed by: Isabelle Capell-Hattam   Group: iGEM15_BABS_UNSW_Australia   (2015-07-26)
Redflag.png

Safety Flag

The iGEM Safety Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety (AT) igem (DOT) org with any questions.

Reason: Invasin

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2017 Safety Page for more information.


pBAD/AraC inducible Invasin

Invasin under an inducible and repressible promoter

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1988
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2605
    Illegal AgeI site found at 979
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None