Composite

Part:BBa_K1676080

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)

Mutant 4 of LDH and wildtype LDP

This construct is an operon for expression of our mutant LDH and wildtype LDP.

The is a mutant of lactate dehydrogenase which contain mutation to these positions: S6Y, R10P, Q14K, L60S, A95D, F126L, K132I, R138P, A177T, W193R, W233S, V271F, T311I.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 797
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2191


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Categories
Parameters
None