Composite

Part:BBa_K1650009

Designed by: Daniel Stukenberg   Group: iGEM15_Marburg   (2015-09-18)

Composite carrying the Lac-inducible CDI-operon,constitutively expressed RFP

Note: This part is similar to BBa_K1650007 except that it does not contain lacI and RFP expression is driven by a constitutive promoter instead of a lac-inducible promoter.
The composite part contains a lac-inducible CDI operon which gives your strain the capability to inhibit growth of bacterial cells that express BamA in their outer membrane (most gram negative bacteria). Additionally the part contains mRFP1 (BBa_E1010) driven by constitutive promoter. IPTG-induced cells harboring BBa_K1650009 express CdiB which forms a beta-barrel structure. The CdiA protein is transported through CdiB and is displayed on the cell surface. When getting in contact with BamA of a target cell, the C-terminal domain is delivered into the target cell acting as pore forming protein thus destroying the proton motoric force of the target cell. BBa_K1650009 could be used to improve transformation efficiency. Induced CDI+ cells would kill false positive cells

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6879
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6879
    Illegal PstI site found at 1325
    Illegal PstI site found at 1510
    Illegal PstI site found at 2005
    Illegal PstI site found at 2080
    Illegal PstI site found at 3112
    Illegal PstI site found at 3259
    Illegal PstI site found at 3398
    Illegal NgoMIV site found at 3133
    Illegal NgoMIV site found at 3628
    Illegal NgoMIV site found at 5203
    Illegal NgoMIV site found at 6757
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
    Illegal AgeI site found at 1768
    Illegal AgeI site found at 2942
    Illegal AgeI site found at 2995
    Illegal AgeI site found at 4030
    Illegal AgeI site found at 4795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2492
    Illegal BsaI site found at 7323
    Illegal BsaI site found at 7744
    Illegal BsaI.rc site found at 2000
    Illegal BsaI.rc site found at 2267
    Illegal BsaI.rc site found at 11005


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