Composite

Part:BBa_K1610302

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-18)


J23100 + RBS + yebF + GFP + Term

This part is used primarily to test the function of yebF. A constitutive promoter and strong RBS is used to produce GFP fused with yebF. This circuit should express GFP that is secreted out of the E. coli membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1065


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Categories
Parameters
None