Coding
SBFP2

Part:BBa_K156010:Design

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-28)

SBFP2 (strongly enhanced blue fluorescent protein)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.


Source

SBFP2 is based on EBFP, which is a mutated variant of GFP (GFP to EGFP to EBFP). The original sequence was obtained from GenBank (VERSION EF030495.1, GI:124264537).

References

Kremers GJ, Goedhart J, van den Heuvel DJ, Gerritsen HC and Gadella TWJ Jr. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46,3775-3783.