SBFP2 (strongly enhanced blue fluorescent protein)
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
SBFP2 is based on EBFP, which is a mutated variant of GFP (GFP to EGFP to EBFP). The original sequence was obtained from GenBank (VERSION EF030495.1, GI:124264537).
Kremers GJ, Goedhart J, van den Heuvel DJ, Gerritsen HC and Gadella TWJ Jr. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46,3775-3783.