Coding

Part:BBa_K156009:Design

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-27)

OFP (orange fluorescent protein)

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.


Source

OFP was cloned from the tentacles of Cerianthus sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).

References

Ip DT, Wong KB, Wan DC. (2007). Characterization of novel orange fluorescent protein cloned from cnidarian tube anemone Cerianthus sp. Marine Biotechnology. 9(4):469-78.