Coding

Part:BBa_K1559001

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-09)

Cas9 and crRNA array

This is basepairs 544 to 5080 of Part:BBa_K1218011(biobricked pCas9), which contains the Cas9 gene and crRNA array. Meant to be used in the assembly of Part:BBa_K1559002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4320
    Illegal BsaI.rc site found at 4297


[edit]
Categories
Parameters
None