Yellow biosafety module for plants
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced. The vector used to perform the assembly was Part:BBa_K1554006, a vector with the BioBricks backbone pSB1A3 and compatible with the Golden Gate-based assembly system GoldenBraid 2.0.
TA29 promoter: Part:BBa_K1554000
T35s: GoldenBraid 2.0 Collection
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