Composite

Part:BBa_K152007

Designed by: David Johnston Monje   Group: iGEM08_Guelph   (2008-10-30)


Synthetic operon of CrtEBIY and FrdBCD and GFP

A limiting factor behind carotenoid accumulation in many organisms is sink strength. Carotenoids are lipophilic, so it is hoped that increased membrane might provide more storage capacity for beta carotene accumulation. In addition to the four Crt genes required for beta carotene production, three of the four genes in the FrdABCD operon were included to increase membrane production, while GFP was added to visualize transcript production and ensure transcription termination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1941
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1478
    Illegal NgoMIV site found at 1608
    Illegal AgeI site found at 732
    Illegal AgeI site found at 4567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6752


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Parameters
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